In this Photoshop tutorial we're going to change the color of the iris that's part of the human eye. It is easy to adjust using a Mask and a Hue & Saturation adjustment layer. Start by making a copy of the background layer, by left clicking on it with the mouse button and dragging it to the New Layer icon ; Photoshop creates a copy of the background layer and makes this new layer active.

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First we have to create a selection. Select the Elliptical Marquee tool and then move your cross hair cursor to the exact middle of the iris.

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Now hold down the Shift + Alt key and hold the left mouse button. Now draw the circle around the iris. Leave some extra space as shown in the example.

With the layer still active, add a mask. Click on the Add Layer Mask icon ,a new mask appears.

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Also notice that a new icon appears in front of the layer that reminds us that we have a layer mask and that the layer mask is active. A layer mask also has a double border if it's active and single one if it's not active.
With the mask selected press the \ key on your keyboard. This will show the mask in a transparent red color. This will make it easier for us to make the necessary adjustments. You can always turn this transparent mask off by pressing the \ key again.

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Go in the menu to Filter? Blur? Gaussian Blur and slightly blur our mask. Use a radius of about 4 pixels.
Normally this should be enough, but we want to include the upper eye lid in our mask, since we don't want to change its color.

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To do that we have to select black as our foreground color and extend the mask by painting on the upper eye lid with a small soft brush . You might also consider to extend the mask around the iris. Paint with white to remove parts of the mask.

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Add a Hue & Saturation adjustment layer. Now hold down the Alt key and click on the New Adjustment Layer icon . Don't release the Alt key yet! Select Hue/Saturation... in the little window that pops up. The New Layer window pops up. Release the Alt key. Select the option 'Use previous layer to create clipping mask' and click Ok. The Hue/Saturation window pops up.

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We're now going to change the hue of the blue colors only, since the iris is blue. Now we slide the hue slider until we have the color that we prefer. You also might consider to adjust the saturation slider.

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